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Click Chemistry Tools dbco-conjugated azdye 488
Dbco Conjugated Azdye 488, supplied by Click Chemistry Tools, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dbco-conjugated+azdye+488/dbco+conjugated+azdye+488/pmc11488284-41-46-52
Average 90 stars, based on 1 article reviews
dbco-conjugated azdye 488 - by Bioz Stars, 2026-09
90/100 stars

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Metabolic Labelling:

Article Title: Antibody-targeted T cells and natural killer cells for cancer immunotherapy
Article Snippet: To metabolically glycoengineer the surface of T and NK-92 cells, cells were seeded at a density of 1 × 10 6 cells per mL in culture medium supplemented with Ac 4 ManNAz (Click Chemistry Tools) for 24 h. To identify the azide group incorporated on the T cell surface, DBCO-conjugated AZDye 488 (Click Chemistry Tools) was incubated with glycoengineered T cells for 30 min at room temperature and quantified by flow cytometry (CytoFLEX Flow Cytometer, Beckman).

Incubation:

Article Title: Antibody-targeted T cells and natural killer cells for cancer immunotherapy
Article Snippet: To metabolically glycoengineer the surface of T and NK-92 cells, cells were seeded at a density of 1 × 10 6 cells per mL in culture medium supplemented with Ac 4 ManNAz (Click Chemistry Tools) for 24 h. To identify the azide group incorporated on the T cell surface, DBCO-conjugated AZDye 488 (Click Chemistry Tools) was incubated with glycoengineered T cells for 30 min at room temperature and quantified by flow cytometry (CytoFLEX Flow Cytometer, Beckman).

Flow Cytometry:

Article Title: Antibody-targeted T cells and natural killer cells for cancer immunotherapy
Article Snippet: To metabolically glycoengineer the surface of T and NK-92 cells, cells were seeded at a density of 1 × 10 6 cells per mL in culture medium supplemented with Ac 4 ManNAz (Click Chemistry Tools) for 24 h. To identify the azide group incorporated on the T cell surface, DBCO-conjugated AZDye 488 (Click Chemistry Tools) was incubated with glycoengineered T cells for 30 min at room temperature and quantified by flow cytometry (CytoFLEX Flow Cytometer, Beckman).



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Click Chemistry Tools dbco-conjugated azdye 488
Dbco Conjugated Azdye 488, supplied by Click Chemistry Tools, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dbco-conjugated+azdye+488/dbco+conjugated+azdye+488/pmc11488284-41-46-52
Average 90 stars, based on 1 article reviews
dbco-conjugated azdye 488 - by Bioz Stars, 2026-09
90/100 stars
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